No. 2

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Possible Answers:

ASST.

Last seen on: –Universal Crossword – Jul 26 2020
LA Times Crossword 11 Aug 19, Sunday

Random information on the term “No. 2”:

No. 2, released as Naming Number Two in North America, is a 2006 New Zealand film written and directed by Toa Fraser in his feature film debut. It was released 16 February 2006 in New Zealand and 3 August 2007 in the U.S. It was adapted from New Zealand-Fijian playwright Fraser’s 2000 play.

Nanna Maria, the matriarch of a Fijian extended family living in a suburb of Auckland, New Zealand, feels that the heart and passion has gone out of her clan. One morning, she demands that her grown grandchildren put on a big family feast at which she will name her successor. The grandchildren—Soul, Charlene, Hibiscus, Erasmus, and her favorite, Tyson—reluctantly turn up, Tyson with his new Danish girlfriend, Maria. Family conflicts play out as the difficult day progresses, but in the end the grandchildren—and eventually Nanna’s children too—join with cousins and others in a traditional celebration.

The film was shot in the Auckland, New Zealand suburb of Mount Roskill during the latter part of summer 2005, with interior scenes of Nanna Maria’s house taking place on a soundstage at Henderson Valley Studios. Post Production was completed in Wellington, at Park Road Post.

No. 2 on Wikipedia

Random information on the term “ASST”:

Rhodanese, also known as rhodanase, thiosulfate sulfurtransferase, thiosulfate cyanide transsulfurase, and thiosulfate thiotransferase, is a mitochondrial enzyme that detoxifies cyanide (CN−) by converting it to thiocyanate (SCN−).

This reaction takes place in two steps. The diagram on the right shows the crystallographically-determined structure of rhodanese. In the first step, thiosulfate is reduced by the thiol group on cysteine-247 1, to form a persulfide and a sulfite 2. In the second step, the persulfide reacts with cyanide to produce thiocyanate, re-generating the cysteine thiol 1.

This reaction is important for the treatment of exposure to cyanide, since the thiocyanate formed is around 1 / 200 as toxic.:p. 15938 The use of thiosulfate solution as an antidote for cyanide poisoning is based on the activation of this enzymatic cycle.

Rhodanese shares evolutionary relationship with a large family of proteins, including

ASST on Wikipedia